Kev Txheeb Xyuas Cov Oxalic Acid Hauv Cov Txiv Hmab Txiv Ntoo thiab Zaub los ntawm High-Performance Liquid Chromatography (HPLC)
1. Lub Ntsiab Cai ntawm Kev Sim thiab Kev Tsim Qauv
Raws li cov organic acid feem ntau hauv cov txiv hmab txiv ntoo thiab zaub, cov ntsiab lus oxalic acid cuam tshuam ncaj qha rau qhov saj thiab cov khoom noj muaj txiaj ntsig. Qhov kev sim no siv cov kua chromatography hloov pauv theem siab (RP-HPLC). Nyob rau hauv cov xwm txheej acidic mobile theem, oxalic acid yog baseline-cais los ntawm cov tshuaj cuam tshuam siv C18 chromatographic kem. Lub tshuab ntes ultraviolet teeb tsa ntawm 210 nm yog siv rau kev tshuaj xyuas ntau, raws li cov yam ntxwv UV nqus ntawm cov pawg carboxyl hauv cov molecules oxalic acid.
2. Kev Npaj Qauv thiab Qauv Qauv
Ib txoj kev gradient dilution siv los npaj cov tshuaj txheem: hnyav 25.0 mg ntawm oxalic acid dihydrate kom raug thiab dilute rau 25 mL nrog dej ntshiab heev kom tau txais 1 mg / mL cov tshuaj npaj. Qhov no raug diluted ua ke rau hauv cov tshuaj txheem ntawm 50, 100, 200, 400, thiab 800 µg / mL, nrog txhua qhov concentration txhaj tshuaj ua peb zaug.
Rau kev npaj cov qauv, kev rho tawm los ntawm lub tshuab microwave tau siv: hnyav 5.00 g ntawm cov txiv hmab txiv ntoo/zaub homogenate, ntxiv 10 mL ntawm 0.1 mol/L hydrochloric acid kua, kho ntawm 60°C nrog lub tshuab microwave rau 10 feeb, lim los ntawm lub lim membrane 0.45 µm, thiab sau cov lim dej rau kev sim.
3. Kev Txhim Kho ntawm Cov Xwm Txheej Chromatographic
Lub theem txawb yog 0.01 mol / L potassium dihydrogen phosphate buffer (pH 2.5) - acetonitrile (95: 5) system, nrog rau tus nqi ntws ntawm 0.8 mL / min thiab kem kub ntawm 35 ° C. Kev kho qhov sib piv ntawm acetonitrile qhia tau hais tias thaum cov theem organic tshaj 10%, lub sijhawm khaws cia ntawm oxalic acid luv dua tsawg dua 3 feeb, tab sis qhov siab tshaj plaws tshwm sim. Nyob rau hauv qhov kawg optimized tej yam kev mob, lub sijhawm khaws cia ntawm oxalic acid yog 4.2 feeb, ua tiav kev sib cais tag nrho ntawm cov citric acid thiab malic acid uas nyob ib sab (kev daws teeb meem> 1.5).
4. Kev Txheeb Xyuas Txoj Kev
Kev txheeb xyuas qhov ntau thiab tsawg ntawm cov kab qhia tau tias muaj kev sib raug zoo ntawm thaj chaw siab tshaj plaws thiab qhov concentration ntawm 10–1000 µg/mL (R² = 0.9993). Qhov txwv ntawm kev kuaj pom (LOD), txiav txim siab los ntawm txoj kev piv ntawm lub teeb liab mus rau lub suab nrov, yog 0.5 µg/mL. Hauv kev sim rov ua dua, RSD rau rau qhov kev txiav txim siab rov ua dua ntawm tib cov qauv zaub ntsuab yog 1.8%. Kev sim rov qab zoo ntawm cov nroj tsuag ntawm peb theem (80%, 100%, thiab 120%) ua rau muaj qhov nruab nrab rov qab zoo ntawm 98.2%, 102.4%, thiab 97.8%, raws li qhov yuav tsum tau ua rau kev tshuaj xyuas ntau.
5. Kev Tshuaj Xyuas Cov Qauv Tseeb
Tau sim rau hom txiv hmab txiv ntoo thiab zaub uas muaj nyob hauv khw: spinach (356 ± 12 mg/100 g), celery (215 ± 9 mg/100 g), txiv lws suav (18 ± 2 mg/100 g), txiv apple (6 ± 1 mg/100 g), txiv tsawb (tsis pom), thiab broccoli (89 ± 5 mg/100 g). Kev sib piv nrog txoj kev ntsuas tus qauv hauv tebchaws (GB 5009.277-2016) qhia tau tias qhov sib txawv ntawm ob txoj kev no tsawg dua 5%, ua pov thawj tias txoj kev no ntseeg tau. Tshwj xeeb, txoj kev HPLC qhia tau tias muaj kev rhiab heev dua li cov txheej txheem titration ib txwm muaj thaum tshuaj xyuas cov qauv qis.
6. Cov Lus Qhia Tseem Ceeb
Tswj xyuas kom tus nqi pH thaum lub sijhawm ua ntej kuaj. Yog tias pH ntawm cov tshuaj rho tawm tshaj 3, cov calcium oxalate precipitation yuav ua rau cov txiaj ntsig kuaj qis. Cov theem mobile yuav tsum tau npaj tshiab txhua hnub kom tsis txhob muaj ntsev precipitation thiab kem thaiv. Tom qab txhua 20 txhaj tshuaj, yaug kem nrog acetonitrile-dej (30:70) rau 30 feeb kom tiv thaiv kev puas tsuaj ntawm kem. Rau cov qauv uas muaj xim (piv txwv li, zaub qhwv ntshav), nws raug pom zoo kom ntxiv cov kauj ruam ntxuav cov khoom khov kho ua ntej txhaj tshuaj.
7. Cov Lus Qhia Txog Kev Siv Thiab Kev Nthuav Dav
Txoj kev no tuaj yeem nthuav dav mus rau kev kuaj pom oxalic acid hauv cov qauv tshuaj lom neeg xws li zis thiab ntshav. Los ntawm kev kho cov khoom sib xyaw ua ke (piv txwv li, ntxiv tetrabutylammonium hydroxide), oxalic acid thiab nws cov metabolites tuaj yeem txiav txim siab tib lub sijhawm. Ua ke nrog lub tshuab ntsuas spectrometry, ib txoj kev kuaj pom tseeb dua tuaj yeem tsim tau. Hauv kev lag luam ua zaub mov, txoj kev no tuaj yeem muab cov ntaub ntawv tseem ceeb rau kev xaiv cov ntau yam oxalic acid qis thiab kev ua kom zoo dua ntawm cov txheej txheem ua noj.
Zibo Anhao Chemical Co., Ltd. - Oxalic Acid
Zibo Anhao Chemical Co., Ltd. yog ib lub tuam txhab tsim khoom thiab xa tawm cov oxalic acid zoo (qib kev lag luam, 99.6% min) los ntawm Shandong, Suav teb. Peb cov oxalic acid yog cov khoom dawb crystalline, tseem hu ua ethanedioic acid, nrog rau cov mis molecular C₂H₂O₄. Nws muaj cov khoom ntshiab siab, tsis muaj kuab paug, thiab ruaj khov zoo thoob plaws ntau pawg, ua tau raws li qhov xav tau ntawm cov ntaub, tawv, metallurgy, kev ua cov av tsawg, kev ntxuav tshuaj, thiab kev tsim kho.
Peb muab ob qho tib si anhydrous thiab dihydrate oxalic acid, muaj nyob rau hauv 25kg hnab, 50kg hnab, thiab 1000kg hnab loj. Nrog rau cov txheej txheem QC nruj, cov ntawv qhia kev xeem tiav, thiab cov phiaj xwm muab khoom yooj ywm, peb ua kom muaj kev xa khoom ruaj khov thiab kev ua tau zoo tas li rau cov neeg yuav khoom thoob ntiaj teb. Peb cov khoom tau raug xa tawm mus rau Southeast Asia, Tebchaws Europe, Africa, thiab Asmeskas, tau txais kev lees paub rau qhov zoo txhim khu kev qha thiab tus nqi sib tw.
Xaiv Zibo Anhao Chemical rau kev muab cov kua qaub oxalic uas ntseeg tau thiab kev pabcuam tshuaj lom neeg ib zaug xwb.
Xaiv Zibo Anhao Chemical rau cov oxalic acid uas muaj cov khoom siv siab thiab muaj txiaj ntsig zoo rau koj txoj kev lag luam. Tiv tauj peb rau kev txiav txim ntau lossis cov lus qhia txog kev siv tshuab: info@anhaochemical.com
